Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some…

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
  1. $5^{\prime}-\mathrm{CGTTCG}-3^{\prime}$ 3'-ATGGTA-5'
  2. 5'-GATATG-3' 3'-CTACTA-5
  3. $5^{\prime}-\mathrm{GAATTC}-3^{\prime}$ $3^{\prime}-\mathrm{CTTAAG}-5^{\prime}$
  4. 5'-CACGTA-3' $3^{\prime}-\mathrm{CTCAGT}-5^{\prime}$

Solution

The palindromic base sequence is a sequence in double-stranded nucleic acids that reads the same on both strands when reading one strand from left to right and the other from right to left (ie, both strands are read $5^{\prime} \rightarrow 3$). Out of the given options $\begin{aligned} & 5^{\prime}-\text { GAATTC-3 } \\ & 3 \text {-CTTAAG-5 } \end{aligned}$ is a palindromic sequence that can be cut at about the middle by particular restriction enzyme.

Asked in: NEET 2010 (Screening)

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