What was the biggest challenge in proving that the codon was a triplet?

What was the biggest challenge in proving that the codon was a triplet?
  1. Deciding the number of nucleotides required to code for amino acids
  2. Determining the biochemical nature of genetic material
  3. Providing proof that the codon was a triplet
  4. Deciphering the combination of nucleotides to code for all 20 amino acids

Solution

The biggest challenge was providing proof that the codon was a triplet.

Asked in: NCERT

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