What is the purpose of incubating purified DNA with restriction enzyme?
What is the purpose of incubating purified DNA with restriction enzyme?
To check the progression of digestion
To prepare recombinant DNA
To charge the DNA molecule
To release DNA from the cell membranes
Solution
The incubation of purified DNA with restriction enzyme helps in cutting the source DNA and the vector DNA, then the cut out ‘gene of interest’ from the source DNA and the cut vector with space are mixed and ligase is added to prepare the recombinant DNA)