What is the function of ligase in restriction enzyme digestion and ligation process?
What is the function of ligase in restriction enzyme digestion and ligation process?
To cut the source DNA
To cut the vector DNA
To prepare recombinant DNA
To purify the DNA
Solution
Ligase is added to the mixture of cut out ‘gene of interest’ from the source DNA and the cut vector with space, which results in the preparation of recombinant DNA)