The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$…

The following diagram showing restriction sites in $E$. coli cloning vector pBR322. Find the role of ' $X$ and ' $Y$ genes:
  1. The gene ' $X$ ' is responsible for controlling the copy number of the linked DNA and ' $Y$ ' for protein involved in the replication of Plasmid.
  2. The gene ' $X$ ' is for protein involved in replication of Plasmid and ' $Y$ ' for resistance to antibiotics.
  3. Gene ' $X$ ' is responsible for recognitions sites and ' $Y$ 's responsible for antibiotic resistance.
  4. The gene ' $X$ ' is responsible for resistance to antibiotics and ' $Y$ ' for protein involved in the replication of Plasmid.

Solution

Correct answer is option (1), because ' $X$ ' in the given diagram is ori while ' $Y$ ' is rop. ' $X$ ' which is ori is responsible for controlling the copy number of the linked DNA and ' $Y$ which is rop codes for protein involved in the replication of plasmid. Options (2), (3) and (4) are incorrect as ' $X$ ' and ' $Y$ ' are not related to these functions.

Asked in: NEET 2024

Practice more Biotechnology - Principles and Processes questions on Aicharya