The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving…
The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?
B-Denaturation at a temperature of about $98^{\circ} \mathrm{C}$ separating the two DNA strands
A-Denaturation at a temperature of about $50^{\circ} \mathrm{C}$
C-Extension in the presence of heat stable DNA polymerase
A-Annealing with two sets of primers
Solution
There are three steps in polymerase chain reaction namely.
(i) Denaturation-at $94^{\circ} \mathrm{C}$ for DNA strand separation.
(ii) Annealing-binding of primer.
(iii) Extension in presence of DNA polymerase which is heat stable.
