The figure below is the diagrammatic representation of the $E$. coli vector pBR 322 . Which one of the given…
The figure below is the diagrammatic representation of the $E$. coli vector pBR 322 . Which one of the given options correctly identifies its certain components (s)?
Ori-original restriction enzyme
rop-reduced osmotic pressure
Hind III, Eco RI-selectable markers
$a m p^{\mathrm{R}}$, tet $t^{\mathrm{R}}-$ antibiotic resistance genes
Solution
Ori represents the site of origin of replication, rop represents the proteins that take part in the replication of plasmid. Hind III, Eco RI are the recognition sites of restriction endonucleases. $a m p^{\mathrm{R}}$ and $t e t^{\mathrm{R}}$ are the antibiotic resistant gene parts.
