Select the correct statement from the following:

Select the correct statement from the following:
  1. Gel electrophoresis is used for amplification of a DNA segment.
  2. The polymerase enzyme joins the gene of interest and the vector DNA.
  3. Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
  4. PCR is used for isolation and separation of gene of interest.

Solution

Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. Restriction endonuclease identifies the specific restriction sites or palindromic sequences and cut the DNA under optimum conditions.

Gel electrophoresis is used for separation of DNA fragments based on size.

PCR is used for amplification of a DNA segment.

The DNA ligase  enzyme joins the gene of interest and the vector DNA.

Asked in: NEET 2020 (Phase 2)

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