Refer to the following statements for agarose-gel electrophoresis : (a) Agarose is a natural polymer…
Refer to the following statements for agarose-gel electrophoresis :
(a) Agarose is a natural polymer obtained from sea-weed.
(b) The separation of DNA molecules in agarosegel electrophoresis depends on the size of DNA.
(c) The DNA migrates from negatively-charged electrode to the positively-charged electrode
(d) The DNA migrates from positively-charged electrode to the negatively-charged electrode.
Choose the most appropriate answer from the options given below :
(a) and (b) only
(a), (b) and (c) only
(a), (b) and (d) only
(b), (c) and (d) only
Solution
Agarose gel electrophoresis is the process of separation of DNA fragments based on their size. As DNA is - vely charged, it moves from negative charged electrode to tve charged electrode inside the Gel Agarose gel shows sieving effect.