Option (3) is the answer because, Kanamycin is an antibiotic which can be used as selectable marker for $E$. coli.
Clal is a restriction site on plasmid pBR322.
Disarmed retroviruses are used to deliver genes into animal cells, which is an indirect method of gene transfer.
Kanamycin ${ }^R$ gene exhibits resistance against kanamycin which is an antibiotic.