Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A…

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell.

B. Cutting of DNA at specific location by restriction enzyme.

C. Isolation of desired DNA fragment.

D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below: 

  1. B, D, A. C
  2. C, A, B, D
  3. B, C, D, A
  4. C, B, D, A

Solution

The process of forming a recombinant DNA molecule involves the following steps:

  1. Isolation of gene ot interest or a desired DNA molecule from an organism.
  2. Restriction digestion of the desired DNA into fragments to insert into the vector. This is carried out by restriction enzymes. 
  3. Isolation of these fragments of DNA (desired fragment) 
  4. The fragments can be separated by gel electrophoresis.
  5. Amplification of the gene by PCR thermo-cycler.
  6. Insertion of recombinant DNA into the host cell.

Asked in: NEET 2023 (All India)

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