Following are the steps involved in the process of PCR. A. Annealing B. Amplification ( $\sim 1$ billion…

Following are the steps involved in the process of PCR. A. Annealing B. Amplification ( $\sim 1$ billion times) C. Denaturation D. Treatment with Taq polymerase and deoxynucleotides E. Extension Choose the correct sequence of steps of PCR from the options given below :
  1. $\mathrm{C} \rightarrow \mathrm{A} \rightarrow \mathrm{D} \rightarrow \mathrm{E} \rightarrow \mathrm{B}$
  2. $\mathrm{A} \rightarrow \mathrm{B} \rightarrow \mathrm{E} \rightarrow \mathrm{D} \rightarrow \mathrm{C}$
  3. $\mathrm{A} \rightarrow \mathrm{C} \rightarrow \mathrm{E} \rightarrow \mathrm{D} \rightarrow \mathrm{B}$
  4. $\mathrm{D} \rightarrow \mathrm{B} \rightarrow \mathrm{E} \rightarrow \mathrm{C} \rightarrow \mathrm{A}$

Solution

The correct answer is option (1) as the correct sequence of steps involved in the process of PCR are : (C) Denaturation (A) Annealing (D) Treatment with Taq polymerase and deoxynucleotides (E) Extension (B) Amplification ( $\sim 1$ billion times). That is $\mathrm{C} \rightarrow \mathrm{A} \rightarrow \mathrm{D} \rightarrow \mathrm{E} \rightarrow \mathrm{B}$ Option (2), (3) and (4) are incorrect as they represent incorrect sequence.

Asked in: NEET 2024 (Re-NEET)

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