During the process of gene amplification using PCR, if a very high temperature is not maintained in the…

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
  1. Annealing
  2. Extension
  3. Denaturation
  4. Ligation

Solution

Denaturation -94-95°C, annealing- 50-60°C, extension- 72°C. Since maximum temperature is required during denaturation, it will be affected the most.

The initial denaturation step is carried out at the beginning of PCR to separate the double-stranded template DNA into single strands so that the primers can bind to the target region and initiate extension.

Asked in: NEET 2021

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